Daisy-KLang

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In this step a short synthetic DNA primers are annealed to the separated strands. Explain two reasons why primers are important in pcr. Polymerase Chain Reaction The thermal cycler takes the. . Previously amplification of DNA involved cloning the segments of interest into vectors for expression in bacteria and took weeks. Annealing Primer to Target Sequence 3. Each of these steps requires a different temperature range which allows PCR machines to control the steps. QUESTION 4 a What are the components reagents required to complete a PCR reaction and what is their purpose. Because DNA polymerase can add a nucleotide only onto a preexisting 3-OH group it needs a primer to which it can add the first. Next an enzyme called Taq polymerase synthesizes - builds - two new strands of DNA. Published July 3 2014. In this step the two strands of double-stranded DNA are separated by heating to 94C for 2 minutes. PCR is...